Calabrian Wine: metagenomics and archeology

3.3.2023

Wine is a crop for which the Calabrian terroir effect has been demonstrated.

The ‘‘terroir concept’’ has become popular in many parts of world. Originally developed for wine, it is now applied to many other quality crops, but surely mainly for wine. Although it is well recognized that vineyard soil is one of the main factors characterizing terroir, it is also well known that soil properties can vary markedly even within a single field, so that a vineyard, for instance, can produce two or more contrasting types of wine. The optimization of agricultural husbandry in relation to soil characteristics is the main focus of Agriculture in Calabria.

METAGENOMICS OF CALABRIAN WINE

In Calabria, a considerable proportion of typical foods, such as dairy products, olive oil, wine, fruits and vegetables, and cereals are of so called “Mediterranean origin”. They are protected by
a ‘denomination of origin’ and the analytical methodologies used for the geographical identification of food products are numerous and for the most part experimental. The most important are DNA analysis and the
chromatographic, spectroscopic, and mass spectrometry techniques.
DNA analysis, using the DNA-barcoding methodology, allows a fingerprint for every product to be identified, guaranteeing its origin and quality.

Another very important perspective for Mediterranean wine and Calabrian one, particularly, is the “environmental” DNA sequences, unique to every species or subspecies, to be utilized like a ‘‘bar code’’ to identify a product by comparing them with a database containing the sequences of all known species. This approach is called “Metagenomics of Wine”

In 2009 a metagenomic experiment was conducted in Sila about potatoes (three types of soil were identified and their related metagenomic markers), the extension of the method to wine is still only a future persepctive. The process is the identification of so-called molecular markers for the traceability of the agricultural food chain, and it can become a new challenge for the protection of high-quality products.

In the world, instead, the last 2 decades have been characterized by an important change in the approaches used for microbial examination, due to the introduction of DNA-based community fingerprinting methods such as DGGE, SSCP, T-RFLP, and ARISA. These approaches allowed for the exploration of microbial community structures without the need to cultivate, and have been extensively applied to decipher the microbial populations associated with the grapevine as well as the microbial dynamics throughout grape berry ripening and wine fermentation.

These techniques are well-established for the rapid more sensitive profiling of microbial communities and these metagenomics approaches to vineyard microbial ecology especially unravel the influence of vineyard management practices on microbial diversity.

ARCHEOLOGY OF WINE AND MEDITERRANEAN WINE-MAKING

A very important attempt to identify the metagenomic origin of wine of rice was done by some Chinese scientists (we are citing the well known article “Metagenomic sequencing reveals the relationship between microbiota composition and quality of Chinese Rice Wine”, Xutao Hong, Jing Chen, Lin Liu, Huan Wu, Haiqin Tan, Guangfa Xie, Qian Xu, Huijun Zou, Wenjing Yu, Lan Wang & Nan Qin, – in Scientific Reports volume 6, Article number: 26621, year 2016).

The recent metagenomic experiment about the wine of rice is very interesting when we remember that the first archeological traces of wine can be actually found in China, in 7,000 B.C., when first type of wine in human history was a fermented mixture of honey, “rice”, grapes and hawthorn berries. Further, rice wine is still a widespread beverage in China today, while the first pure wine of grapes can be dated to 6,000 BC and geographically placed in the region of Georgia and the Caucasus in general.

Recent news of 2023 refer that the origin of the grape and that of the wine, which have so far been an unsolved mystery, date back even 11 thousand years ago, thanks to two domestication events geographically separated by more than 1,000 kilometers but similar in the result.
They occurred in Western Asia and the Caucasus region. It was reconstructed by the largest genetic analysis ever conducted, which examined over 3,000 samples of vine varieties also coming from private collections and specimens never documented. From that area the wine making, then, spread to the Phoenician, Greek and Latin world…

Map of Vitis vinifera (Neolithic period). Image Credit: Science journal

The study is published in the journal Science, also conquering the cover, by the international group led by the Chinese Agricultural University of Yunnan, the State Laboratory of Agricultural Genomics in Shenzhen and the Chinese Academy of Sciences in Beijing, with the Italian collaboration of the Universities of Milan, Milan- Bicocca and Mediterranea of Reggio Calabria, of the National Center for Biodiversity (Nbfc) of Palermo and of the National Research Council (Cnr).

Particularly, the Chinese scientists investigated the influence of microbial composition on the quality of rice wine, and sequencing was performed for 110 wine samples on bacterial 16S rRNA gene and fungal Internal Transcribed Spacer II (ITS2).

Bioinformatic analyses demonstrated that the metagenomics of rice wine is marked by Lactobacillus brevis. These results led to a conclusion that metabolisms of microbes influence the wine quality and can mark it.

PERSPECTIVE: CALABRIAN OENOLOGY AND WINE ARCHEOLOGY

The new techniques of Metagenomics are a new wide open field for proving the best quality of Calabrian soils and of their vineyards. Further, archeology of wine can avail of this method to investigate history of wine in the south of Italy, as environmental heritage of Phoenician, Greek and Latin history into Calabrian wines.

Calabrese “Scirubbetta”, the oldest ice cream in the World

25.1.2023

The main ingredient is snow… the purest one, but not the one that has just fallen, but the one called “ciciarusa” in jargon , which is snow that has turned into grains of ice due to the alternation of daytime heat with night frosts, collected in pristine spaces of our beloved Calabria.

The Calabrian scirubbetta , or snow mixed with fig honey or cooked must, is a beautiful custom that is still very much alive in Calabria when the white winter flakes arrive. A preparation as simple as it is ancient, whose origin is lost in the mists of time and is to be considered as the first ice cream in history.

Snow Flakes, the ingredient

AN ANCIENT TRADITION

The name scirubetta comes from the Turkish “sharbat” which means “beverage”, a term in turn derived from the Arabic “sharbat”, from which the Italian words “syrup” and “ sorbet” also originate. Sharbat in the Middle East is a sweet drink served very cold, of various textures to be savored by the spoonful.

A profound tradition that opens on the primordial form of ice cream and traces of which can be found in any population that has had access to snowy mountain areas. A sort of ice cream for the poor.

The mother-recipe: Sharbat

The traditional preparation of the scirubetta involved the collection of snow in a large pot from which it was then redistributed directly into glasses or cups or into a centerpiece tray in which the magical and generous encounter with the sweet fig honey took place, elsewhere popularly called vincotto di fichi or simply cooked with figs, prepared by housewives in the last part of summer.

In some areas of Calabria cooked must was also used. In more recent times, but still daughters of the ancient oriental tradition, the variants of scirubetta di neve with orange juice (winter fruit by definition) or lemon and sugar, or again with coffee and sugar, or finally with chocolate.

Cooked must for scirubetta

THE ICE CREAM ARCHETYPE

The definition of granita is inadequate, since it was made through the processing of long slabs of ice, using a special machine. It is not even a sherbet, as it is made with the addition of milk and other ingredients. Scirubetta is, in truth , the oldest form of ice cream and, probably, Calabria was one of the first regions of southern Italy (thanks to its mountain ranges) where this preparation was experimented and conceived for the first time.

Suffice it to say that about a century ago, in the historic center of Cosenza there was a street called the “vineddra da nivi” (today Via Spirito Santo) where the snow collected in the Sila was sold to allow citizens to savor the scirubetta, rigorously prepared with cooked fig honey (better known as fig honey) or, alternatively, cold coffee or orange juice.

Cosenza by night

A KEY INGREDIENT: FIG HONEY

The fig honey is a 100% natural condiment, obtained by boiling figs, and still made today, following the ancient Cosenza recipe, through a long manual process that involves the use only of Cosenza DOP figs and water, without addition of preservatives, dyes or thickeners. The yield is very low considering that around 25kg of figs are needed to obtain 7 litres.

Specifically, the figs are boiled in water for more than 12 hours after which they are pressed, process which takes place in the press, the extract obtained is then brought to the boil again for about 8 hours or in any case until it takes on a dark color and the characteristic smell. “The secret for a top quality honey is to use raw materials coming only from the best local crops and work them, strictly by hand, according to the ancient recipes jealously guarded only by the best producers.

Scirubetta figs honey

In addition to the preparation of the scirubetta and the typical Calabrian sweets ( turdilli , scalille , etc.), its use in the kitchen is vast. It is delicious on ice cream, cheese or fruit salad. But its flavor makes it a perfect condiment also to accompany meat and fish dishes.

It is also important to underline the therapeutic properties of this precious and sweet liquid, used since ancient times as an expectorant, to cure coughs and colds.

Scirubetta, in its enviroment